Anti-CTIP2/BCL11B; clone 25B6

Code: MABE1045 D2-231

Application

Immunohistochemistry Analysis: A 1:240 dilution from a representative lot detected CTIP2/BCL11B in mouse brain tissue sections.

Immunofluorescence Anal...


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Application

Immunohistochemistry Analysis: A 1:240 dilution from a representative lot detected CTIP2/BCL11B in mouse brain tissue sections.

Immunofluorescence Analysis: A representative lot detected CTIP2 localization in 4% paraformaldehyde-fixed floating embryonic (E14 & E16) and postnatal (P3 & P6) mouse brain sections (Arlotta, T., et al. (2005). Neuron. 45(2):207-221).

Western Blotting Analysis: A representative lot detected doublet CTIP2 bands, representing two spliced isoforms, in Jurkat nuclear extracts as well as in SIRT1 immunoprecipitate (Senawong, T., et al. (2003). J. Biol. Chem. 278(44):43041-43050).

Anti-CTIP2/BCL11B Antibody, clone 25B6, Cat. No. MABE1045, is a highly specific rat monoclonal antibody that targets CTIP2 and has been tested in Immunofluorescence, Immunohistochemistry, and Western Blotting.

General description

B-cell lymphoma/leukemia 11B (UniProt Q99PV8; also known as B-cell CLL/lymphoma 11B, BCL-11B, COUP-TF-interacting protein 2, mRit1, Radiation-induced tumor suppressor gene 1 protein) is encoded by the Bcl11b (also known as Ctip2, Rit1) gene (Gene ID 58208) in murine species. CTIP2/CBL11B is a C2H2 zinc finger protein that binds GC-rich response elements and is reported to associate with a variety of cofactors, including the corepressor complexes NuRD, Sirt1, histone acetyltransferase (HAT) p300, functioning both as a transcriptional repressor and activator. CTIP2/CBL11B is required at multiple developmental checkpoints for T cell commitment and maintenance. CTIP2/CBL11B expression is initiated at double-negative 2 (DN2) stage of T cell development and is maintained in mature T lymphocytes, with lower levels found in NK cells, but not in B cells, myeloid or dendritic cells. Bcl11b-/- mice die shortly after birth due to a blockade of thymocyte development at the DN2 stage. Bcl11b-deficient DN2 thymocytes are highly proliferative and retain the potential to differentiate into NK cells and myeloid lineages, but not mature T-cells.

Immunogen

GST-tagged recombinant full-length mouse CTIP2 Beta isoform (Senawong, T., et al. (2003). J. Biol. Chem. 278(44):43041-43050).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 150 mM NaCl, 15 mM HEPES, pH 7.5, with 0.02% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CTIP2/BCL11B in 10 µg of HEK293 cell lysate.

Specificity

Clone 25B6 recognizes both the Alpha and Beta spliced isoforms of human and mouse CTIP2/BCL11B.

Target description

~96 kDa observed. 95.52/94.57 kDa (human/mouse isoform Alpha) and 88.48/87.34 kDa (human/mouse isoform Beta) calculated. Uncharacterized bands may be observed in some lysate(s).

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcerat
clone25B6, monoclonal
Gene Informationhuman ... BCL11B(64919)
isotypeIgG2a
NCBI accession no.NP_067374
Quality Level100
shipped inambient
species reactivitymouse, human
technique(s)western blot: suitable, immunohistochemistry: suitable (paraffin)
UniProt accession no.Q99PV8
This product has met the following criteria to qualify for the following awards:



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